FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46186010
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG505920.10953966363407447No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN474530.10274323328644325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31250.018.06986426
GGCATAG174550.015.76049435
ATAGGGG180450.015.61869138
AGCTTAG176850.015.54809345
GGAGGTC245800.014.3310284
GAGCTTA198400.014.00494844
CATAGGG199050.013.92155437
GTCACTC245150.013.8355578
TGTCGTT42450.013.77771828
GGGAGGT271550.013.7161343
TGGCATA204050.013.69148434
ACTCTCC253300.013.39272411
GGTCACT254850.013.3442657
GCATAGG209950.013.29540736
ATATAGC53100.012.7982976
GAGGTCA286000.012.3747085
GTCGTTC46550.012.32197829
GCTAAGT47900.012.3128514
AGGTCAC295900.012.0385926
TCGTTCC49950.011.70953830
Produced by FastQC (version 0.11.5)