FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51664740
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4030320.7800910253298478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3195280.6184643530578108No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1972360.3817613327774416No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1247580.24147610149591386No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1149660.22252313666922546No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA568580.11005184580431451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA286300.022.96499345
CATGCAT161700.019.3954939
GGAGGTC406900.018.4967174
ATATAGC66950.018.3504246
GGGAGGT413050.018.1172853
TATATAC168700.017.6461547
GTTGTCG62450.017.61752326
GAGGTCA438800.017.4037025
AGCTTAG363200.017.27246945
AAACTCT395850.016.92749244
AAAACTC395900.016.84586543
ATATATA151650.016.454826
ATATACA194150.016.0742388
TTCCATA201850.016.0509092
ATACATG202000.016.04046
TACATGC200300.015.8620417
TGCATTC196850.015.82997511
TATAGCT80050.015.684777
GGCATAG405750.015.5832135
GCTAAGT71850.015.56330714
Produced by FastQC (version 0.11.5)