FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52978216
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29950.022.1613566
TCGACGA34800.019.072747
ACCGCGT57800.017.7114736
CGGTATA50350.017.24885220
GACCGCG60200.017.0801335
CGACGAG39000.016.0380128
AGACCGC65650.015.66220954
TTCGGCG74200.015.25258544
CCGCGTT67600.015.0772397
TCGGCGC74450.014.86897445
CGCGTTC70200.014.5829248
CAGACCG74450.014.4757943
CGTACGC81450.014.0604195
CCGTACG82600.013.8374234
AACGCTA94500.013.42818123
ACGCTAC96150.013.19774524
GTCCGTT42850.013.1378681
CGCCCCG87550.012.900825523
TACGCCA90750.012.7682377
GTCGGTT48650.012.6824571
Produced by FastQC (version 0.11.5)