FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51969521
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1082800.20835289207302873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT217100.024.41784745
ACCGCGT243950.022.8291876
GACCGCG245450.022.6805085
AGACCGC249250.022.4339924
CCGCGTT252650.022.0252597
CGCGTTC258650.021.5665078
CAGACCG264900.021.2529643
AGAACGA262050.020.24252744
ATACGGA281050.018.90809233
AATACGG281650.018.82517232
TACGGAG282100.018.7997234
TCAGACC309200.018.4625952
AAGAACG291850.018.20793543
GCGTTCT312550.017.9624049
CAATACG297650.017.93971431
ACGGAGA314350.016.98102235
TTCGACG16150.016.8520776
CTCAGAC379350.016.5329021
AACGCTA110050.016.16908823
ACGCTAC111900.015.94263824
Produced by FastQC (version 0.11.5)