FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51969521
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28000.011.33156826
ATAGGGG119550.011.27221938
AGCTTAG122800.010.86678745
GGCATAG125350.010.55427335
GGGAGGT215850.09.7054593
CATAGGG141150.09.38856637
TGTCGTT36200.09.13770828
GCATAGG149350.09.08400536
GAGCTTA149450.09.0779844
GGAGGTC223900.08.8104774
ATATAGC46450.08.5225436
TGGCATA157500.08.51420234
ACTCTCC229600.08.35757911
GTCACTC231850.08.1878538
GGTCACT225950.08.13299757
TCAGACG74600.07.99300342
CGTAGGG59400.07.992099337
GGCGTAG61600.07.926004435
GCGTAGG60950.07.89960436
TATAGCT49550.07.85314137
Produced by FastQC (version 0.11.5)