FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44843507
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1177540.26258873999306076No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG903000.2013669448288244No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG792800.17679259563708966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT377050.016.6272323
AGCTTAG286000.016.44671445
GGAGGTC373700.016.3055784
GAGGTCA385500.015.9230695
GGTCACT386850.015.4659367
GTTGTCG27100.015.35873426
GGCATAG311600.015.2564235
GTCACTC391300.015.23821458
AGGTCAC405600.015.0781696
ATAGGGG323150.014.92686838
ACTCTCC408400.014.69198911
GAGCTTA327050.014.44541544
TGGCATA335500.014.2961534
TGGGAGG355450.014.0248232
TCACTCT435750.013.8325559
CTGGGAG422500.013.1978371
CACTCTC451300.013.14152210
CACCGAA477700.012.42972845
CATAGGG384700.012.38652137
AGGGATC194700.012.264552
Produced by FastQC (version 0.11.5)