FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50974779
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG818710.16061079931312697No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT770700.15119241615544815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC87150.019.5230274
ACGTTCG17200.019.2241926
AACGCTA234100.018.54451823
ACGCTAC235550.018.4017124
ACCGCGT94050.017.9614076
GTTAGTT204300.017.7925344
CGCTACC244000.017.69990725
CAGACCG99450.017.448783
TCACGCT261550.017.1572368
AAAACGC257050.016.96757721
GACCGCG99950.016.9325925
GCACTAC89650.016.71027225
CGTGGTT264050.016.5007141
GAAAACG266100.016.36515420
GTCCGTT33650.016.3016031
AAACGCT268000.016.22395122
TCGGCGC148300.016.19582245
GCCGGCT277450.016.1504611
GTTTTCG267650.016.1208745
GCGTACG9650.016.08353223
Produced by FastQC (version 0.11.5)