FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50974779
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG835620.16392812610330296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG268750.015.66367845
GGGAGGT396250.014.8074013
GGAGGTC409950.013.9497374
GGCATAG305050.013.83039835
TCAGACG136750.013.5572062
GAGCTTA312250.013.55216244
TGGGAGG338150.013.1875662
TGGCATA321650.013.15166834
ATAGGGG327700.013.02536138
ACCCGGC82950.012.9906565
GTTGTCG23550.012.89908126
ACTCTCC448950.012.4721911
ATAGCTC84050.012.07383839
CATAGGG373000.011.47362237
TCACCCG97600.011.410273
TAGCTCT90150.011.3064540
GGTCACT508150.011.1651647
TAGCGTA8950.011.0618737
ATATAGC33300.011.0117256
GTCACTC524450.011.0112748
Produced by FastQC (version 0.11.5)