FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54160063
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG650000.12001463144531424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA169950.020.40244323
ACGCTAC170450.020.38242124
CGCTACC177650.019.4930125
TCACGCT198700.018.0595688
AAAACGC196300.017.75388121
AGACCGC105300.017.4568824
GAAAACG205050.017.13890520
ACGTTCG22200.017.126886
GTCCGTT50000.016.9790971
TTCGACG21750.016.6537176
AAACGCT211600.016.51304622
TACGGTA79500.016.13100644
CAGACCG116450.016.075653
GCCGGCT239350.016.050511
CGTGGTT217900.016.01435941
GCGGACT106250.015.9883132
CGGACTT116600.015.6883283
GCGTATG106400.015.60672638
TTCACGC234250.015.5973577
ACGGTAC83250.015.3771945
Produced by FastQC (version 0.11.5)