FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52682713
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35000.011.570168526
AGCTTAG140600.010.724647545
GGCATAG141400.010.5962435
ATAGGGG145100.010.45033538
ATATAGC53200.09.4326656
GGGAGGT231400.09.4220893
CATAGGG159850.09.41552637
GGAGGTC223400.09.1856964
TGTCGTT45250.09.14800128
GGTCACT212200.08.9615397
GCATAGG169600.08.95374936
GTCACTC218700.08.7263128
TATAGCT57950.08.6600967
GAGCTTA176200.08.55680744
AGGTCAC236050.08.427326
TGGCATA189350.08.12676734
TCAGACG83400.08.1201052
GCTAAGT51050.08.110814
ACTCTCC246900.08.085114511
TAGGGGA232000.08.07796939
Produced by FastQC (version 0.11.5)