FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49431368
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG566480.11459929654384642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG180900.016.21717545
GGCATAG182200.015.93934835
GTCACTC261700.015.7881518
ATAGGGG188800.015.66628138
GGTCACT272150.015.066327
CATAGGG194100.015.03017437
AGGTCAC296100.014.4028236
GAGCTTA208900.014.21250444
TGGCATA206950.014.19653334
ACTCTCC295400.014.04703311
GGGAGGT319150.013.973933
GAGGTCA310800.013.8038455
GGAGGTC307650.013.7493074
GCATAGG212400.013.67185636
TAGGGGA328100.012.45627539
GCGTAGG93500.012.12480336
GGCGTAG94200.011.86856435
CGTAGGG94200.011.84350937
TCACTCT356750.011.8146469
CACTCTC370450.011.19560310
Produced by FastQC (version 0.11.5)