FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49647863
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT634170.1277335944952958No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG499420.10059244644628511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT212150.019.6714271
TCACGCT218450.019.3923238
CCGGCTT232500.019.026412
TTCACGC226150.018.7917377
TTCGACG20950.018.577776
ACGCTCA244650.017.42589610
CGGCTTC279600.016.8916473
CACGCTC255950.016.691739
GTTAGTT195400.016.49957844
GCACTAC95800.016.20650925
GTCCGTT45050.016.1651691
AAAAACG107900.015.51371721
GAAAACG161450.015.38492420
CCCGTAT35500.014.7978991
AACGCTA197250.014.20201523
TACGTGC74450.014.11431733
GCGGACT79100.014.0807242
CCCAGAT208050.014.0662871
CGCATTA43250.014.04565520
ACGCTAC200350.014.027204524
Produced by FastQC (version 0.11.5)