FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54011948
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG803960.14884854736215034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG212250.018.66727345
GTTGTCG38050.018.27153626
GGCATAG236900.016.69727335
GAGCTTA251000.015.91086944
ATAGGGG257300.015.443377538
TGGCATA263250.015.05949434
TGTCGTT46950.014.80806128
CATAGGG268050.014.723337
GCATAGG279400.014.21378336
GGGAGGT397550.014.2113283
GGAGGTC394000.013.7166884
ATATAGC58100.013.3989026
ACTCTCC406500.013.11213311
GCTAAGT54300.012.8875414
TCGTTCC58100.012.19853630
GTCACTC445250.012.0619558
TATAGCT64700.011.9625417
GGTCACT443300.011.9576777
GTCGTTC59450.011.77014429
ACCCGGC89350.011.6841175
Produced by FastQC (version 0.11.5)