FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48797921
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG617870.126618099160413No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA541220.1109104627633624No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG523510.1072812097876055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG185400.016.72318545
GGCATAG202900.015.12228635
ATAGGGG208500.014.82301338
GAGCTTA220600.014.07510944
CATAGGG229000.013.41746437
GTCACTC295000.013.3922838
TGGCATA233400.013.26264434
GGGAGGT318200.013.1589363
ACTCTCC310500.012.8683311
GCATAGG242300.012.69959136
GGAGGTC331250.012.35544
GGTCACT320000.012.2969537
AGGTCAC335550.012.222726
GAGGTCA343750.011.892945
ATATAGC40100.011.54476456
GTTGTCG32800.011.45817226
TCACTCT361200.011.1741989
TAGCGTA9000.010.76260837
CACTCTC368900.010.67290810
AGGGATC153600.010.6670632
Produced by FastQC (version 0.11.5)