FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40329502
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC44450.028.04193532
CGTCCCG48300.026.08620335
TCGTATG51050.024.63689845
GTCCCGA61850.020.29852336
GTCCGTT31600.020.0302771
ACACGTC66150.019.35358813
CGTCTGA67900.019.11987916
ACGGTAC93200.018.29882645
ACGTCTG71550.018.20742815
CCGATCT66100.018.07436439
TCCCGAT69150.017.89535937
CCCGTAT26700.017.632051
TGCGCGT16150.017.55367345
ATGCGCG16550.017.40130844
GTCCGAT22300.017.3736611
ACGTTCG14400.016.874516
CCCGGAT83100.016.805771
TCACCCG75250.016.68390330
CCCCCCT27750.016.3966871
CTCGTAT75100.016.32775744
Produced by FastQC (version 0.11.5)