FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63345310
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG757220.11953844728204818No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG687150.1084768548768646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG221650.016.02304535
AGCTTAG229150.015.66568345
GGTCACT485650.015.3761617
GTCACTC498600.015.0670218
GGGAGGT528900.014.9719273
ATAGGGG243750.014.81522738
TGGCATA246250.014.38585434
ACTCTCC532400.014.27535611
GGAGGTC548350.013.9944314
TAGCGTA12300.013.81428437
AGGTCAC559400.013.7504136
GAGCTTA266000.013.70645944
GAGGTCA565850.013.6731545
TCACTCT586750.012.9987569
AGCATAG300800.012.95792945
CACTCTC594400.012.67288110
ATAGCTC108700.012.42170839
TCAGACG119600.012.096342
TAGGCTA103950.011.90824310
TATCCTA109650.011.8420285
Produced by FastQC (version 0.11.5)