FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51440240
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG700530.13618326819626037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG240250.014.60134245
GGAGGTC311150.014.4048834
GGGAGGT326650.014.3560313
ATAGGGG258350.013.60325838
GGCATAG253950.013.57417635
GTCACTC338650.013.07478
GGTCACT339350.013.0015357
GTTGTCG38250.012.76690626
ACTCTCC354350.012.51339411
GAGGTCA376600.012.1875985
GAGCTTA288550.012.146893544
TGGCATA293100.011.94569634
AGGTCAC391250.011.7311886
CATAGGG301600.011.48125837
GCATAGG307250.011.29955436
CACTCTC395300.011.21755210
TCACTCT414550.010.9897159
TGTCGTT46500.010.98582828
AACTCTA135100.010.7926945
CTGGGAG401500.010.3353881
Produced by FastQC (version 0.11.5)