FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53376698
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA144350.021.48109423
ACGCTAC147850.021.0030224
CGCTACC150300.020.69067625
AAAACGC165000.019.0473421
TCACGCT172600.018.8597778
GAAAACG172900.017.94169620
CCGGCTT204400.017.8409462
TTCACGC185950.017.7596447
GCCGGCT193200.017.3723241
AAACGCT185250.017.01386522
ACGCTCA195000.016.77407610
GTCCGTT38950.016.4822391
CCCGTAT34750.016.270381
CACGCTC207150.015.9531229
CGTGGTT199250.015.57059841
GTTTTCG208500.014.68213945
GCGGACT70350.014.5500012
CGGCTTC274000.014.4667553
TCGTGGT219550.014.37684440
CGAACGA52850.014.34767832
Produced by FastQC (version 0.11.5)