FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53700281
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG848630.1580308304159526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC138400.023.42644
GACCGCG140600.023.0598415
AACGCTA217450.022.85295923
ACGCTAC217300.022.7762424
CGCTACC221450.022.22760825
ACCGCGT146650.022.0885456
CAGACCG151250.021.4513073
AAAACGC248200.020.02995721
TTCGACG21200.019.3117776
GAAAACG258800.019.1052620
AAACGCT261700.019.01418922
CCGCGTT174400.018.5480867
CGTGGTT274850.018.08048641
TCGTGGT282050.017.49920340
GTTTTCG280800.017.44608945
CGCGTTC186800.017.3409278
TCAGACC204300.016.4104482
GCTACCT323450.015.70518726
CTACCTC328300.015.36353127
TCACGCT339100.015.2907898
Produced by FastQC (version 0.11.5)