FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49427548
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1018190.20599646173020764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG238800.019.93210845
GGCATAG276600.017.11528835
ATAGGGG292350.016.30115338
GAGCTTA295000.016.23404744
GTCACTC387450.016.0911278
GGTCACT390250.015.9180377
CATAGGG308800.015.45454337
TGGCATA314900.015.204802534
GTTGTCG31800.015.13987826
ACTCTCC420700.014.93132711
AGGTCAC432450.014.8020736
GAGGTCA437700.014.7815525
GGAGGTC434900.014.7368744
GGGAGGT460100.014.6291463
GCATAGG326100.014.59318836
TCACTCT462500.013.6403439
TAGGGGA469800.013.28645539
CACTCTC473600.013.22109810
ACCCGGC91800.012.894425
TGTCGTT37600.012.86420228
Produced by FastQC (version 0.11.5)