FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52366946
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1435050.2740373670062791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT273800.025.87358545
GACCGCG310950.022.9504035
ACCGCGT311850.022.9130156
AGACCGC315100.022.6909644
CCGCGTT321950.022.1662627
CGCGTTC326600.021.830018
AGAACGA326750.021.77635444
CAGACCG331350.021.7410623
TCAGACC366950.019.9444222
AATACGG357100.019.79267932
TTCGACG21750.019.6474046
ATACGGA360550.019.61715733
TACGGAG361700.019.54725534
AAGAACG366700.019.36036143
CAATACG372150.018.95868531
GCGTTCT377700.018.924219
ACGGAGA400300.017.72040235
CCCAATA414050.017.25079729
CTCAGAC459650.017.213931
CGGAGAG423700.016.65623936