FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45695803
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG104250.015.0007723
TAGCGTA13750.014.39782737
ACCCGGC107400.014.3937035
CACCCGG122450.012.9367994
ATAGCGT16150.012.67611936
ACGGACA126350.012.6630618
CGCCGTA16150.012.5368245
CGGACAC124100.012.29429713
GCGTCGT42100.011.59901311
CCGGACA135800.011.58305712
CACGGAC130800.011.49256217
CGGACAG131000.011.44029619
AGCTTAG89800.011.34852245
ATAGCTC130600.011.041594539
TCGTGTA44000.010.99596814
CGGCCCG143500.010.9296738
GTCGTGT45100.010.77762213
GACACGG139650.010.68368815
GGCATAG92550.010.64671535
GCGTATA20050.010.54703639
Produced by FastQC (version 0.11.5)