FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44097793
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1555010.35262762469768044No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT685130.15536605199267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT370900.026.9826241
TCACGCT378500.026.9775928
CCGGCTT432550.024.4668922
TTCACGC419900.024.4302487
ACGCTCA425100.024.3046110
GAAAACG373150.024.16222420
AACGCTA386250.023.8617523
ACGCTAC387950.023.72238724
CACGCTC432750.023.6722349
CGGCTTC459950.023.5523073
CGCTACC397500.023.10771825
AAAACGC407950.022.6694921
AAACGCT424800.021.78105722
GTTTTCG432300.020.60876545
TCGTATG29150.020.3342245
GGCTTCA542650.020.2436644
AGAAAAC455900.019.99272319
CGCTCAG526700.019.79137611
CGTGGTT470750.019.61791641
ACGTCCG30650.019.3093532
Produced by FastQC (version 0.11.5)