FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46161321
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1433810.310608528729063No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT904850.1960190870620882No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC535420.115988881687333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG46400.027.6572726
CGGTATA55950.027.23399520
GCCGGCT330250.026.6658081
TCGACGA49700.025.9986427
CGACGAG51700.023.5830368
CCGGCTT414950.023.2401642
GTTCGAC52350.023.121455
TCACGCT428550.022.6057198
ACCGCGT52100.021.6213916
TTCACGC451150.021.5467417
CGGCTTC475650.021.0870193
CACGCTC462400.020.960429
ACGCTCA474500.020.71918110
AACGCTA461400.020.58936923
ACGCTAC462900.020.58470324
GACCGCG55100.020.5644455
GAAAACG454150.020.4173920
AAAACGC475200.020.07046321
CGCTACC473050.020.06365225
AAACGCT490800.019.40552522
Produced by FastQC (version 0.11.5)