FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42299504
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG568880.13448857461780167No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT466200.11021405830196024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32600.028.072856
ACCGCGT97750.026.5919846
GACCGCG103300.025.1927285
AGACCGC106350.024.7663234
CCGCGTT105650.024.646137
TCGACGA39100.023.9810927
CGCGTTC110300.023.68828
CGGTATA51600.022.8780120
CAGACCG119700.022.2296543
CGACGAG42400.021.6899688
GTTCGAC44300.020.9695195
GAACGAT131550.019.488645
TACGGAG132750.019.47387734
AACGCTA172050.019.28066323
AATACGG136250.019.14012132
ACGCTAC173700.019.059124
ATACGGA138700.018.86529233
CGCTACC177950.018.54125
TCAGACC150850.018.5193652
GCGTTCT146450.017.8716669
Produced by FastQC (version 0.11.5)