FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40192077
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT33300.017.4529691
TGCGCGT30750.017.1321245
CGATTAG36050.015.9192284
ACGGTAC98400.015.65128445
TACGGTA100400.015.47347544
AACGCTA127250.015.36664123
ATGCGCG34450.015.29209644
CCCGTAT27150.015.2076371
CTACACG53250.015.19763245
ACGCTAC129000.015.15879924
CGCTACC133900.014.571156525
CTCCGGT53900.014.4878611
TTACGGT107350.014.4090743
CTCGGTT74500.014.2769621
ACGTTCG18450.014.215986
CCTTACG101050.013.98066841
AAAACGC142500.013.89294121
CTCCGAT43800.013.8325871
CTTACGG112950.013.75421842
TCGCGTG6250.013.6298432
Produced by FastQC (version 0.11.5)