FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46797575
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG704730.15059113639969593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36750.022.1607466
AACGCTA207900.021.11624323
ACGCTAC208000.021.10744524
CGCTACC212850.020.55279425
TCGACGA41450.020.190067
GCCGGCT213750.019.9484121
CCGGCTT241050.019.7771592
AAAACGC222450.019.74314321
TTCACGC235100.019.1762757
TCACGCT235350.019.1750248
GTCCGTT40000.019.1462081
AAACGCT235600.018.72842822
CGGTATA63800.018.47864720
GAAAACG237950.018.47559520
GTTCGAC44400.018.3427835
ACGCTCA254750.017.62647810
CGACGAG46450.017.3871338
CACGCTC261450.017.2091969
CGGCTTC294100.016.982363
CTCCGGT59100.016.1600321
Produced by FastQC (version 0.11.5)