FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54756826
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35750.025.9914446
ACCGCGT45950.023.1596496
TCGACGA43850.020.9331747
CGACGAG44800.020.2381828
CCGCGTT52050.020.2287757
TGCGCGT45500.019.8817245
AGACCGC53950.019.5184654
GACCGCG54850.019.2801025
CGGTATA69300.018.92793320
ATGCGCG48050.018.82660144
CGCGTTC57700.018.014028
AACGACG21250.017.25735916
TCGGCGC100900.017.19501145
ATGACGG73050.016.90821616
TTCGGCG104900.016.81821444
CCCGTAT37050.015.9795851
CAGACCG67550.015.8885813
AACGCTA107750.014.74233923
ACGAGGA85350.014.68236910
ACCGCGA28650.014.53069545
Produced by FastQC (version 0.11.5)