FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47113038
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT612030.12990671499469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG42450.028.4605796
TCGACGA48950.024.6353787
CGACGAG50050.022.9701568
CGGTATA76700.021.97132120
TCACGCT210200.021.60988
ACGTCCG40000.020.9264732
AACGCTA224600.019.68581423
ACGCTAC227450.019.42991424
ACGCTCA232050.019.36172710
ACGTTCG20000.018.898466
CGCTACC235350.018.75904725
AAAACGC240200.018.5095221
CACGCTC245100.018.3859149
TTCACGC250000.018.3315077
TCGTATG47700.017.64014645
GAAAACG250950.017.60856620
GTCCGTT41900.017.578031
CAGTTCG74500.017.515353
ATGACGG94200.017.33990516
GTTCGAC71550.016.5709765
Produced by FastQC (version 0.11.5)