FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55009882
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT72400.018.265276
GACCGCG71600.017.8455945
AGACCGC73100.017.6035374
ACGCTAC153800.016.08509624
AACGCTA153950.015.99638223
CCCGTAT34950.015.8042881
CCGCGTT84250.015.6428197
CGCTACC159950.015.34008825
GTCCGTT40100.014.9552371
CAGACCG89250.014.87323
TCACGCT175050.014.8133928
ACGGTAC79750.013.72774845
AAAACGC183500.013.56749621
CCGGCTT218550.013.5519842
CCTTACG81400.013.44105241
ACGCTCA197000.013.40260510
CGCGTTC99400.013.3265058
CTACACG61550.013.13893345
GCCGGCT205350.013.0210511
TTCACGC204200.012.9851037
Produced by FastQC (version 0.11.5)