FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55009882
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29650.015.25991126
AGCTTAG146700.012.80435445
GGCATAG149750.012.30749535
ATAGGGG159200.012.09873338
TGTCGTT41900.011.44290528
GGGAGGT268500.010.8525623
TCACCCG94100.010.7604323
GAGCTTA176150.010.75152444
CATAGGG174800.010.68475137
ATATAGC50950.010.6402766
GGAGGTC259850.010.3644314
TGGCATA180800.010.35547534
GTCACTC254700.010.350518
ACGGACA93450.010.3030518
GCATAGG179150.010.28717336
GGTCACT256250.010.1829357
ACTCTCC262400.010.02781111
ACCCGGC100750.09.77955
TATAGCT56400.09.6918357
TCAGACG80800.09.6076892
Produced by FastQC (version 0.11.5)