FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54349416
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT842390.15499522570766905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT179350.022.16969345
AGACCGC196600.020.2632034
ACCGCGT198550.020.0524026
GACCGCG199050.020.0137945
CGCGTTC208800.019.1002438
CCGCGTT209550.018.9890827
CAGACCG217200.018.6308043
AGAACGA222350.018.07513644
ATACGGA231800.017.17938433
AATACGG233050.017.10659432
TACGGAG238400.016.7790634
AAGAACG247750.016.13410643
CAATACG252500.015.89584431
TCAGACC257650.015.8803912
CCCGTAT36250.015.5754251
TTCGACG23150.015.5464776
GCGTTCT264750.015.2251329
AACGCTA103500.015.19158523
ACGCTAC104750.014.96805524
GTCCGTT42500.014.8727631
Produced by FastQC (version 0.11.5)