FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52783379
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT118050.020.5218056
GACCGCG126150.019.2399225
CCGCGTT126450.019.1585357
AGACCGC128450.019.0880474
GAACGAT131750.018.61634345
CGCGTTC134850.017.998338
CAGACCG139650.017.6534713
AATACGG153150.015.80664432
CCCGTAT36500.015.7766561
ACGTTCG24850.015.4787376
TACGGAG157300.015.38940234
ATACGGA159300.015.182240533
AGAACGA161150.015.13657844
TCAGACC173550.014.8531042
TTCGACG15250.014.160136
TCGGCGC66750.014.15847545
TTCGGCG67450.014.11195544
CAATACG182950.013.39185231
AAGAACG182650.013.34501543
GTCCGTT43150.013.0846111