FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48889977
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT552280.11296384942050595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32400.026.5875056
TCGACGA37150.023.663687
CGGTATA46600.022.17351720
GACCGCG151550.021.7309995
ACCGCGT152550.021.7188636
AGACCGC158750.021.0375924
CGACGAG39050.020.81548
CAGACCG170900.019.8004323
CCGCGTT167400.019.7789947
CGCGTTC169050.019.5990098
GAACGAT183550.018.37756345
GTTCGAC50250.016.9671635
TCAGACC207850.016.8968242
AATACGG214000.015.49300132
GCGTTCT215250.015.4847469
ATACGGA215700.015.32993533
ATGACGG63700.015.27849816
CTCAGAC265400.015.2090041
AGAACGA222400.015.19707444
AACGCTA199800.015.18970123
Produced by FastQC (version 0.11.5)