FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51787356
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT949150.1832783276288521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT188850.024.7818945
ACCGCGT211850.022.2081326
AGACCGC214550.021.9811734
GACCGCG217350.021.594445
CCGCGTT222750.021.1114037
CAGACCG226200.020.9385973
CGCGTTC225900.020.8164398
AGAACGA232900.020.15268544
ATACGGA242250.019.3096633
AATACGG243500.019.21053332
AACGCTA85500.019.10473423
TACGGAG244850.019.0220434
ACGCTAC87650.018.66199124
TCAGACC264600.018.3528462
CAATACG264200.017.81610531
GCGTTCT272800.017.336239
CGCTACC93950.017.26688625
AAGAACG274550.017.10376243
ACGGAGA276200.017.00969935
ACGTTCG19300.016.4379716
Produced by FastQC (version 0.11.5)