FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51787356
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG133450.012.9968945
ACCCGGC95400.012.0340825
GGCATAG147500.011.80462435
GTTGTCG24450.011.5017926
ATAGGGG157400.011.39086838
ACGGACA102150.011.36638618
ATAGCTC94650.011.31308839
TCACCCG109700.011.2442873
CATAGGG164300.010.65264437
GAGCTTA169000.010.48922944
CGGACAC111800.010.34566513
CCGGACA118050.010.29411912
GGGAGGT283400.010.1425933
GCATAGG174400.010.06154736
TGGCATA176550.09.87424934
TAGCTCT110250.09.71235340
ACACGGA115450.09.60943916
TGATAGC117600.09.4309437
CACCCGG127550.09.3885374
GGAGGTC269850.09.3675094
Produced by FastQC (version 0.11.5)