FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46173519
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT530140.11481472746315913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG25700.025.0373526
AACGCTA191950.021.43800423
ACGCTAC192050.021.4034124
TCACGCT208700.020.601488
CGCTACC200350.020.52817325
TCGACGA33950.019.4170747
TTCACGC221900.019.4163427
ACGCTCA225950.018.90889710
GAAAACG226650.018.10628120
AAAACGC228200.018.07203121
CACGCTC242000.017.822439
AAACGCT239000.017.29298622
GTTTTCG238000.016.71719245
CGGTATA54350.016.10312820
CGTGGTT256850.016.05934341
CCGGCTT190400.015.9526092
GTTCGAC41250.015.8720995
CTACCTC269450.015.70615527
TCGTTAC21150.015.64103444
CGACGAG39350.015.6091098
Produced by FastQC (version 0.11.5)