FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62785213
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1181790.18822744138814979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA293550.022.09905423
ACGCTAC293600.022.05697324
CGCTACC302350.021.36700825
TTCGACG28400.021.3078466
TCACGCT320300.020.9578888
AAAACGC316350.020.61998621
GAAAACG331900.019.5520320
GCCGGCT345550.019.5058651
TTCACGC348300.019.4345447
CCGGCTT378750.019.0262182
ACGCTCA361600.018.69991110
AAACGCT350150.018.68741822
CACGCTC361450.018.6018719
CGGCTTC412750.018.0966913
CGTGGTT385250.016.92329241
TCGTGGT384250.016.82008240
GTTTTCG379850.016.7572645
TCGACGA38350.016.4247327
CTACCTC411050.016.04512227
CCCGTAT43800.015.9282061
Produced by FastQC (version 0.11.5)