FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62785213
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG704120.11214742554110631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG239550.014.72808545
GGCATAG261300.013.5190135
GGGAGGT378600.012.7670383
GAGCTTA282200.012.69318744
GGAGGTC366750.012.6200964
GTCACTC352400.012.5210338
ATAGGGG288550.012.47757738
GGTCACT354850.012.4282437
CATAGGG295700.012.084290537
GAGGTCA389000.012.0316645
TGGCATA301900.011.91708434
CACCGAA276300.011.67789745
AGGTCAC393250.011.660956
ACTCTCC382000.011.597811
GCATAGG306250.011.59368236
GCACCGA292150.010.98242644
TCACTCT426600.010.6807729
CACTCTC425250.010.37067610
TAGGGGA443450.09.96109239
TGGGAGG392850.09.8985952
Produced by FastQC (version 0.11.5)