FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47034671
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT629860.13391398017857933No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT526440.11192594501192535No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC488780.10391908556137237No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG482370.10255626110364416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27950.025.5148356
ACCGCGT115650.024.976686
GACCGCG117450.024.4981255
AGACCGC122650.023.587874
CAGACCG133100.022.0908723
CCGCGTT132150.021.8070687
TCGACGA34000.021.437867
CGGTATA40450.021.35642220
TCGGCGC128450.020.28438445
CGCGTTC145550.019.8921478
GTTAGTT160400.019.58257544
CGACGAG35450.019.4821768
TTCGGCG134550.019.348144
GTTCGAC37750.019.0698975
CGTACGC142400.018.6575765
CCGTACG143050.018.6042524
TCAGACC166900.018.2101822
AACGCTA263250.017.854523
CGCTACC181650.017.75091725
ACGCTAC265350.017.69755724
Produced by FastQC (version 0.11.5)