FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54395140
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1318730.242435261679628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG368050.017.7007245
GGGAGGT534100.016.3693183
GAGGTCA533850.016.2913745
GGAGGTC533300.016.141014
AGGTCAC547650.015.6807686
GTCACTC544400.015.6642418
GGCATAG417550.015.57570935
GGTCACT550150.015.4746797
GAGCTTA428450.015.31048644
ATAGGGG425900.015.30643738
ACTCTCC577700.014.71024211
TGGCATA447600.014.64729334
TGGGAGG461600.014.6311022
TCACTCT613600.014.0369769
ACCCGGG135650.014.033372
CACCCGG144750.013.5548281
CACTCTC636950.013.3498710
CATAGGG509200.012.89453237
CTGGGAG594350.012.4257781
GCATAGG529400.012.40678436
Produced by FastQC (version 0.11.5)