FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49140917
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT61750.022.3351756
TGCGCGT58350.021.94003145
AGACCGC67500.020.4658784
ATGCGCG63700.020.41522844
GACCGCG67250.020.4081365
CCGCGTT68300.020.1932187
CGCGTTC70200.019.5825798
TTCGACG21950.019.4753326
TTCGGCG82600.018.60400644
CAGACCG77550.018.1617773
TCGGCGC85050.017.98872845
AACGCTA63650.017.24992623
ACGCTAC66000.016.49936524
CGTACGC98100.015.96268755
CCGTACG97900.015.9493354
CTCCGTT75200.015.8528631
GAACGAT89050.015.7405545
CGGTATA36100.015.33181920
AATACGG90800.015.28872732
CGCCCCG102400.015.2704123
Produced by FastQC (version 0.11.5)