FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53823003
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1176630.21861099054617966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG292450.019.74638445
GGCATAG331900.017.47082935
GAGCTTA344300.016.81531744
GTCACTC447550.016.7924068
ATAGGGG348900.016.74368138
GGTCACT462100.016.2285377
TGGCATA360800.016.16887934
CATAGGG371100.015.80194237
AGGTCAC486000.015.7082026
ACTCTCC485600.015.574911
GAGGTCA499700.015.3644175
GGGAGGT513800.015.2364533
GCATAGG389200.015.06250436
GGAGGTC521550.014.6868334
TCACTCT538450.014.1198439
TGGGAGG438800.013.8964432
CACTCTC550600.013.76735610
TCTCCCC596950.012.6412613
AGGCTCT475700.012.62800720
GCTCTGT598950.012.59305422
Produced by FastQC (version 0.11.5)