FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58540852
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG646460.11042886769054881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG239450.014.87750745
ATAGGGG252900.014.26836138
GGCATAG250300.014.164803535
GGGAGGT342100.014.0259243
GGAGGTC328150.013.6838114
GAGCTTA267100.013.26888244
GTTGTCG32200.012.92469526
TGGCATA291750.012.20633434
ACTCTCC371300.012.12217511
GTCACTC367150.012.0813758
CATAGGG294950.012.05862937
GGTCACT367500.011.9894827
GCATAGG304300.011.70288836
CACTCTC415450.010.77977810
TGGGAGG367350.010.4774832
TAGGGGA424800.010.3639439
GAGGTCA467000.010.0884485
GCTCTGT454750.010.00692322
TCACTCT459050.09.9863459
ACCCGGC61100.09.7950125
Produced by FastQC (version 0.11.5)