FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48040277
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1480770.3082351086360306No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT610980.12718078207584024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT305300.024.8946761
CCGGCTT361500.023.0731952
TCACGCT354000.022.624728
TTCACGC367350.022.0046087
AACGCTA367800.021.52154523
ACGCTAC369650.021.41994524
CGCTACC374600.021.08303825
AAAACGC391050.020.2924721
ACGCTCA399450.020.17433410
CACGCTC399700.020.1561079
GAAAACG395450.020.03254920
CGGCTTC426500.019.9946613
AAACGCT402350.019.72270222
CGTGGTT405250.019.27500541
GTTTTCG414500.018.81327845
TCGTGGT431250.018.21725740
GCGGACT105250.017.7225342
AGACCGC60300.017.6497154
CTACCTC458550.017.45893127
GACCGCG60900.017.3641575
Produced by FastQC (version 0.11.5)