FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48040277
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG720020.1498784030741538No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA633540.1318768415927327No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG495180.10307600849179116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30350.016.01683426
GGGAGGT329900.015.3169183
AGCTTAG249300.015.08232645
GGAGGTC337400.014.8604984
ATAGGGG277250.013.5760238
GGCATAG277950.013.49257335
ACTCTCC363850.013.232553511
GTCACTC371500.013.2086638
GAGGTCA395300.012.9773055
GGTCACT379450.012.9554687
TGGGAGG308650.012.8735222
GAGCTTA295300.012.82425344
ATATAGC41100.012.639626
TGGCATA303250.012.45620734
AGGTCAC406900.012.4298436
TCACTCT423950.011.7744759
CACTCTC412100.011.7414110
TGTCGTT41100.011.49952628
CATAGGG336250.011.28118337
TCAGACG101600.011.1611712
Produced by FastQC (version 0.11.5)