FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46370125
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG131800.015.63383245
GGCATAG151850.013.37752135
ATAGGGG157350.013.25294538
GAGCTTA168250.012.40732844
CATAGGG166600.012.31457137
GGGAGGT212350.011.8786243
GCATAGG172700.011.86664136
GTCACTC204400.011.7902818
GGTCACT210250.011.3980147
TGGCATA185900.011.060616534
GCGTCGT31900.011.00500611
GGAGGTC231250.010.7521114
AGGTCAC238650.010.5790816
ACTCTCC235600.010.24898311
GAGGTCA254150.010.14635
TAATCGG23350.09.8293894
TAGGGGA243700.09.77555239
ACCCGGC68600.09.7091425
TCACTCT264300.09.5098059
CACTCTC260350.09.23086510
Produced by FastQC (version 0.11.5)