FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54543392
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG553980.1015668405807985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG212050.013.71372745
GTTGTCG33150.013.50540626
GGGAGGT306450.013.12896253
ATAGGGG230400.012.72329738
GGCATAG236150.012.38492935
GTCACTC299900.012.2765628
GGTCACT307450.012.0262387
GGAGGTC322650.011.9123614
AGGTCAC327400.011.6227736
GAGCTTA252800.011.56183544
ACTCTCC329150.011.45212611
TGGCATA270050.011.03018334
CATAGGG270600.010.88302337
GAGGTCA359550.010.8149895
GCATAGG274600.010.64257536
ATATAGC52650.010.2578826
TGGGAGG321350.09.7962512
TCACTCT385900.09.7622649
TGTCGTT48100.09.49488828
CACTCTC389550.09.49170610
Produced by FastQC (version 0.11.5)