FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51758295
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1060940.20497970421939132No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC631370.1219843118866261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38900.024.175736
ACCGCGT224750.022.5835556
GACCGCG228050.022.2578165
AGACCGC232400.021.9864864
CCGCGTT234100.021.6623427
CAGACCG242650.021.178333
TCGGCGC156550.021.13191645
CGCGTTC243450.020.8306358
TTCGGCG160400.020.7087244
GAACGAT251050.020.49229445
TCGACGA48450.019.5961887
TCAGACC283250.018.6433262
CGGTATA59850.018.5318920
AATACGG289050.017.8315532
CGACGAG50850.017.6096428
GCGTTCT294900.017.2875339
GTTAGTT155000.017.23416744
ATACGGA298850.017.17905833
TACGGAG303900.016.86397734
AGAACGA309600.016.71853344
Produced by FastQC (version 0.11.5)