FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46274071
Sequences flagged as poor quality0
Sequence length51-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC11050.026.81537664-65
ATTGCCG44150.025.3549160-61
ACGTCTG646750.025.22348494-95
CGTCTGA596300.024.7211594-95
ACACGTC751450.023.72445594-95
CACGTCT721350.023.68749694-95
CACACGT820750.023.61662794-95
CTACGCC13750.023.3456366-67
GCACACG904700.023.10824694-95
GCCCTAA42800.022.886452-53
GCCGACC48200.022.7116364-65
ACCCCTA32750.022.61877470-71
TGCACTA115750.022.54289256-57
TGCCGAC49850.022.45552662-63
GTCGGAT57850.021.91432248-49
AGAGCAC1193850.021.88240494-95
TTCGACG25700.021.8808066
GAGCACA1167200.021.4864794-95
AGCCCTA57750.021.09599750-51
GTTCCGT124550.020.88243994-95
Produced by FastQC (version 0.11.5)